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Citation:
Thibodeau R, Bell D, Jones J, Grocott-Gomori methenamine silver stain. Reference article, Radiopaedia.org (Accessed on 19 Mar 2025) https://doi.org/10.53347/rID-173597
Grocott-Gomori methenamine silver (GMS) stain is a histological stain frequently used to screen specimens for fungal organisms. The procedure relies on the presence of polysaccharides along the fungal cell wall.
During the staining process, chromic acid is first applied to the specimen which oxidizes the polysaccharides. This produces aldehydes. Grocott alkaline hexamine-silver solution is then applied, which reduces silver ions to black amorphous silver 1.
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1. Morris GB, Ridgway EJ, Suvarna SK. Traditional stains and modern techniques for demonstrating microorganisms in histology. In: Suvarna SK, Layton C, Bancroft JD, editors. Bancroft's Theory and Practice of Histological Techniques. 8th ed. Elsevier: 2018. p. 267.
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2. Gomori G. A New Histochemical Test for Glycogen and Mucin*. Am J Clin Pathol. 1946;16(11_ts):177-9. doi:10.1093/ajcp/16.11_ts.177 - Pubmed
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3. Grocott R. A Stain for Fungi in Tissue Sections and Smears. Am J Clin Pathol. 1955;25(8_ts):975-9. doi:10.1093/ajcp/25.8_ts.0975 - Pubmed
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