Last revised by Craig Hacking on 17 Jan 2024

The myocardium defines the middle layer of the cardiac wall between the endocardium and the pericardium and forms the muscular part of the heart.

The myocardium represents the middle layer of the cardiac wall. It is located between the endocardium and the epicardial layer of the pericardium within the walls of the cardiac chambers arranged in different sheets wrapped around in different orientations.  

The left ventricular myocardium can be subdivided into the following layers or zones from inside to outside:

  • subendocardial
  • midmyocardial
  • subepicardial

The exact myocardial architecture has been somewhat controversial. It has been compared to a contractile complex three-dimensional mesh, made up of myocytes merging with their neighboring cells in a nonuniform anisotropic manner supported by an extracellular collagenous matrix-forming bundle of myofibres, which are configured in a helical or spiral pattern within the two ventricles 1-4.

The ventricular myocardium is thicker than the atrial myocardium, in particular, the myocardium of the left ventricle.

The inner border merges with the subendocardial tissue layer, which contains collagen, elastic fibers small blood vessels and nerves as well as the Purkinje fibers and connects the extracellular matrix of the myocardium to the endocardium 4.

The outer border is formed by the subepicardial layer, which is interconnected to the epicardium, the latter consisting of a lining of mesothelial cells, a subserosal layer of connective tissue and a variable amount of epicardial fatty tissue.

The arterial supply of the myocardium is provided by the coronary arteries.

The vascular territories of the left ventricular myocardium are divided into 17 segments and illustrated by the cardiac segmentation model of the American Heart Association (AHA) 6,7.

Venous drainage of the myocardium is provided via the cardiac veins and the coronary sinus as well as the Thebesian veins 8.

Cardiac lymphatic flow passes from the endocardium through the myocardium to the epicardium where small lymphatic vessels drain into larger collecting vessels. In this process, myocardial contractions help to advance lymph flow. The lymphatic function is critical to maintaining the myocardial interstitial fluid equilibrium and cardiac function and it has been advocated that it aids tissue repair and prevents adverse remodeling in case of myocardial injury 9.

The myocardium is innervated by the cardiac conduction system. Myocardial conduction happens from cardiomyocyte to cardiomyocyte via the intercalated disks, which form the mechanical and electrical contacts between the myocardial cells 10.

The myocardium consists of cardiomyocytes grouped in strands also known as myofibres and the surrounding extracellular matrix with endomysial and perimysial components. The cardiomyocytes are linked with each other through distinctive junctional compounds, the intercalated discs, which facilitate intercellular electrical impulse conduction. The myocyte strands diverge in different angles from the lining of the epicardial surface 1-5.

The myocardium can be depicted and evaluated with ultrasound e.g. echocardiography, cardiac CT and cardiac MRI.

In most imaging techniques the myocardium displays a muscle-like appearance.

Echocardiography has been traditionally used as a first-line imaging technique in the evaluation of cardiac morphology and function as well as in the assessment of myocardial contractility and the contractile reserve 11.

More recently it has been also used for the evaluation of myocardial strain or simplified for the assessment of regional deformations of the myocardium such as thickening, shortening or lengthening. Echocardiographic strain imaging techniques include tissue Doppler imaging and speckle tracking 12.

Cardiac CT can be used for the assessment of myocardial morphology and myocardial perfusion. In addition, research has been conducted concerning the use as an alternative for myocardial extracellular volume quantification 13,14.

The myocardium can be visualized and is routinely evaluated with a whole series of sequences and imaging techniques assessing its function and inherent tissue properties, such as 15-18:

Specific less often used cardiac imaging techniques for the evaluation of the myocardium include:

In addition, the myocardial architecture and myofibril ultrastructure has been investigated with diffusion tensor imaging. Due to long acquisition times and this imaging technique has been mainly used in research 3.

SPECT and PET imaging and is used in myocardial perfusion imaging and the assessment of myocardial viability. PET permits the measurement of absolute myocardial blood flow. Furthermore, it can be used in the evaluation of myocardial inflammation 19.

The word myocardium is derived from the Greek words 'myo-' muscle and 'kardia' heart. 

The following pathologies and diseases are related to the myocardium:

and many more...

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Cases and figures

  • Figure 1: LV myocardial segmentation and coronary artery territories
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  • Figure 2: LV myocardial segments (diagram)
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