Quadruple screening test

Last revised by Daniel J Bell on 18 Apr 2022

The quadruple screening test, also known as the quad screen, AFP Plus quad test or multiple marker screening test, is a maternal antenatal screening blood test that can be used in conjunction with other investigations e.g. ultrasound soft markers, to estimate the risk of aneuploidy 1

This is especially valuable when no first-trimester screening is available. It has been proved as a feasible and efficient non-invasive method for screening for Down syndrome. It is usually used before more invasive tests such as amniocentesis 2.

Temporal window: fetal age 17-20 ± 2 weeks.

It tests for the three substrates of the triple test (alpha-fetoprotein, beta human chorionic gonadotropin, unconjugated estriol) plus inhibin-A.

As with the triple test, it helps counsel patients for high-risk pregnancy and progressive assessment for the validity of other ultrasound soft markers 3.

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