Agenesis of the corpus callosum - 3D reconstruction of Probst bundles

Case contributed by Dr Francesco Sciacca


Agenesis of the corpus callosum. Hydrocephalus, intellectual impairment and spasticity.

Patient Data

Age: 65 years
Gender: Male

3D reconstruction result of Probst bundles and Cingulum in a Patient (male) with agenesis of corpus callosum.

Case Discussion

In patients with agenesis of the corpus callosum the tractography has been shown to be able to objectivate connection fibers atypical secondary to the failure of corpus callosum along the midline. These fibers are called Probst bundles.

Data acquisition

A 1.5 T Philips Achieva was used. DTI data were acquired using a single-shot echo planar imaging (EPI) sequence with SENSE parallel imaging * (EPI factor = 59). Spin Echo technique (TR = 11090 ms; TE = 107 ms; Flip Angle = 90°; nex = 2). The imaging matrix was 112 x 112, with a field of view of 224 x 224 mm. The image orientation was axial, with a 2.0 mm slice thickness (slice gap = 0 mm), which was aligned parallel to the anterior-posterior commissure line. A total of 60 slices covered the entire cerebral hemispheres and the brainstem. The diffusion weighting was encoded along 32 indipendent orientations, with a maximum b-value = 800 s/mm2. The scanning time of one dataset was about 12 min. A co-registered T1-weighted 3D Gradient Echo was also recorded for anatomical guidance.

* SENSE parallel imaging were used to reduce the length of the EPI-train, thereby reducing susceptibility related distortions.

Reconstruction of tracts

The 3D tract reconstruction was performed using the FACT (Fiber Assignment by Continuous Tracking) method. Fractional Anisotropy (FA) threshold = 0.18.


A. Politi, MD and G. M. Latagliata, MD.

Disclosure: I, Francesco Sciacca, have no actual or potential ethical or financial conflict of interest in relation to this device. This case is not intended to be a personal endorsement or recommendation of this product.

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Case information

rID: 68778
Published: 22nd Jun 2019
Last edited: 24th Sep 2019
Inclusion in quiz mode: Excluded
Institution: sins

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