Small cell carcinoma of lung

Case contributed by Dr Mikkaela McCormack


A 62-year-old man presented to his general practitioner (family medicine / primary care physician) complaining of haemoptysis. Sputum analysis was negative when performed via his general practitioner. He was referred to the lung outpatient clinic and then proceeded to bronchoscopy, where bronchial brushings were performed and material sent to pathology for cytological examination. His smoking status and past history were unknown to pathology.

Patient Data

Age: 62
Gender: Male

Cytology Images

Background proteinaceous fluid and benign bronchial epithelial cells with occasional small clusters of malignant epithelial cells displaying pleomorphic, hyperchromatic nuclei with granular chromatin, inconspicuous nucleoli and irregular nuclear membranes with scant cytoplasm.  Nuclear molding is focally present.

Diagnosis:  Positive for malignancy - features in keeping with small cell carcinoma

Case Discussion

The Case:
Whilst there wasn't an abundant amount of material in this case, there were enough groups of malignant cells displaying the characteristic features of small cell carcinoma to confidently make this diagnosis.  In addition, tissue biopsy specimens taken from the patient showed similar features further confirmed the diagnosis (though this  cytology case was reported on its own merits).  

It is extremely important when making a definitive diagnosis of small cell carcinoma within the lung to do so appropriately, and only when you have the right amount of evidence and confidence to do so, as this may be the only tissue diagnosis the patient ever receives, and can dictate treatment pathways. 

Things to remember when considering a diagnosis of small cell carcinoma in a cytology setting:

Cell Size
Better preserved tumor cells may look deceptively larger than you're expecting - in spite of its name, small cell carcinoma can have varying cell sizes, and some well preserved or air dried cells can look larger than you may think.  That said, if all cells are very large (especially when comparing them to benign bronchial epithelial cells, or inflammatory cells), think twice before making a diagnosis of small cell carcinoma.

Cell Cytoplasm
If cells have a moderate to abundant amount of cytoplasm, think very carefully before making a diagnosis of small cell carcinoma.   Even larger 'small cell' cells shouldn't have much cytoplasm. Small cell characteristically has a scant amount of delicate cell cytoplasm exhibiting a basophilic hue.

Prominent Nucleoli
Similarly, if cells display prominent nucleoli - think twice before making a small cell diagnosis - small cell should only have small/inconspicuous nucleoli - remember their neuroendocrine links and that they should have a similar salt and pepper chromatin pattern.

Nuclear Molding
Nuclear molding needs to be just that: true molding.  Nuclei need to be wrapped around each other and deforming/conforming to the shapes of their neighboring nuclei - like a big nuclear 'group hug', as I like to think of it.  Cells that are simply hanging around next to each other (i.e. merely being cohesive) are not molding.

Crush Artefact
Again, this is a feature we often find as a striking red flag in histology specimens but that may be absent in a cytology preparation.  If you see it, it may help, but it may not, as this feature may also be caused by over enthusiastic smearing of slides.

Apoptotic Debris, Mitotic Activity
These are also pieces of evidence you can use when making your diagnostic case, but aren't definitive in and of themselves.

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Case information

rID: 44031
Published: 3rd Apr 2016
Last edited: 14th Aug 2019
System: Chest
Inclusion in quiz mode: Included

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